Journal: Biochimica et biophysica acta

Article Title: Signaling pathways of cell proliferation are involved in the differential effect of erythropoietin and its carbamylated derivative.

doi: 10.1016/j.bbamcr.2013.04.006

Figure Lengend Snippet: Fig. 2. Expression of EpoR and βcR under different stages of cell differentiation. A) TF-1 and UT-7 cells were subjected to erythroid differentiation by 30 μM hemin during 48 h (H) while differentiation of neuronal SH-SY5Y cells was achieved by retinoic acid (10 μM RA, 4 days). Cell extracts were resolved by SDS-PAGE (T = 10%), electroblotted on a NC membrane and then immunodetected by chemiluminescence using specific antibodies. Each bar represents mean ± SEM of band density with respect to β-actin, used as control of sample loading. B) SH-SY5Y cell incubations with 250 ng/ml of either Epo (E) or cEpo (cE) for 10 min and p-Jak2 analyzed by flow cytometry. C) After 18 h without growth factors, TF-1 cells were incubated with 20 ng/ml Epo or 20 ng/ml cEpo during 10 min and p-Jak2 was detected. D) UT-7 cell cultures were incubated with 20 ng/ml of either Epo or cEpo (10 min) to determine Jak2 phosphorylation (p-Jak2). Each bar represents mean ± SEM. Significant differences of fluorescence intensity: **P b 0.01; * P b 0.05 (n = 3).

Article Snippet: Antiphosphorylated FOXO3a, anti-FOXO3a, and anti-phosphorylated Jak2 antibodies were from Cell Signaling Technology.

Techniques: Expressing, Cell Differentiation, SDS Page, Membrane, Control, Cytometry, Incubation, Phospho-proteomics